DAVID GRANOVSKY

Posts Tagged ‘myelin’

SALT in FAST FOOD = IMMUNE DISORDERS? MULTIPLE SCLEROSIS?

In ALL ARTICLES on September 16, 2015 at 6:39 pm

In the last article we discovered that we don’t know everything there is to know about the human body.  Far from it.

“In contradiction to decades of medical education, a direct connection has been reported between the brain and the immune system….It seems astonishing that, after centuries of dissection, a system of lymphatic vessels could have survived undetected.” via https://repairstemcell.wordpress.com/2015/09/16/system-connecting-brain-to-immune-system-found/

So what about processed foods?  Could the foods you are eating be a contributing factor to immune diseases like Multiple Sclerosis?

A study [Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cells ] showing that “mice fed with a high-salt diet develop a more severe form of (experimental autoimmune encephalomyelitis) EAE, in line with augmented central nervous system infiltrating and peripherally induced antigen-specific TH17 cells.”  In other words, “…increased dietary salt intake might represent an environmental risk factor for the development of autoimmune diseases through the induction of pathogenic TH17 cells.”  via 

In the latest study, scientists discovered that exposing these cells in a lab to a table salt solution made them act more ‘aggressively.’  They found that mice fed a diet high in refined salts saw a dramatic increase in the number of Th17 cells in their nervous systems that promoted inflammation.  They were also more likely to develop a severe form of a disease associated with multiple sclerosis in humans.  The scientists then conducted a closer examination of these effects at a molecular level.  Laboratory tests revealed that salt exposure increased the levels of cytokines released by Th17 cells 10 times more than usual. Cytokines are proteins used to pass messages between cells.  via 

More and more we realize that all the systems of the body are connected and everything we eat and do and don’t eat and don’t do has an affect.

Bare this in mind the next time you decide to “treat yourself” with some junk food.

Milan, Italy study used stem cells derived from mouse skin tissue to treat MS – Lima multiple sclerosis | Examiner.com

In STEM CELLS IN THE NEWS on November 5, 2013 at 2:25 pm
Milan, Italy study used stem cells derived from mouse skin tissue to treat MS

 

An experiment was published Tuesday in Nature Communications by researchers at San Raffaele Scientific Institute in Milan and the University of Milan in Italy, that showed they were able to reduce nervous system damage in mice.

Milan, Italy study used stem cells derived from mouse skin tissue to treat MS – Lima multiple sclerosis | Examiner.com.

RESEARCHERS DISCOVER KEY TO STEM CELL THERAPY FOR MS PATIENTS

In STEM CELLS IN THE NEWS on November 30, 2012 at 9:00 am

 clinical trial capsules

We know stem cells work on MS, we just hadn’t figured out exactly how yet…and now we have!

– DG

CLEVELAND, Ohio — One of the most promising and exciting treatment avenues for multiple sclerosis is the use of a patient’s own stem cells to try to stop — or even repair — some of the disease’s brain tissue damage.

But injecting a patient with a dose of his or her own bone-marrow stem cells was actually a pretty crude method of treating the disease, because no one was quite sure how or why it worked. Last year, doctors at the Cleveland Clinic, University Hospitals Seidman Cancer Center and Case Western Reserve University began trying this for MS patients in a Phase 1 clinical trial after positive results were seen in mice.

Multiple sclerosis is an autoimmune disease in which the immune system attacks the myelin sheaths that surround and protect nerve cells. When myelin is damaged, the nerve cells are exposed and unable to do their job, which is sending signals to the brain and back. This results in the loss of motor skills, coordination and cognitive abilities.

Like many other researchers using stem cells, the local group didn’t know exactly how their treatment worked, but they knew that when they gave these human mesenchymal stem cells, or MSCs, to mice with a mouse version of the disease, the mice got better.

Figuring out why the mice improved could help researchers see if the MSC injection will work well in a particular patient before the patient is injected, and possibly augment or improve the treatment as well.

In May, the research group at CWRU, headed up by neurosciences professor Robert Miller, discovered exactly what it is in the stem-cell soup that has a healing effect: a large molecule called hepatocyte growth factor, or HGF. The team published their results in Nature Neuroscience.

Miller’s group knew that it could be the stem cells themselves, by coming in physical contact with the myelin damage, that were having a healing effect. Or it could be something the stem cells secreted into the surrounding liquid culture, or media, they were grown in, that was key. HGF is secreted by the stem cells, Miller said.

The team identified the HGF by first injecting only the liquid the stem cells were grown in, but not the stem cells themselves, into the mice they were studying. The mice got better, so the team knew whatever was helping was in the media.

Next, they isolated the small, medium and large molecules from the media and tried each size on the mice. Only the large-molecule treatment had the healing effect, meaning that whatever was helping was somewhere in that mix, Miller said.

“The molecule that jumped out at us was HGF,” he said, because it is the right size, is made by MSCs, and in a couple of studies had been shown to be involved in myelin repair.

So the scientists took a purified sample of HGF and injected it into the sick mice. They got better. When they blocked the receptor for HGF in the mice, they stayed sick. It was pretty compelling evidence that they’d found what they’d been looking for, Miller said.

“We went on to show that HGF, like the MSCs, is regulating both the immune response, and it is independently promoting myelin repair in the brain,” he said.

MSCs, taken from the bone marrow, are currently being tested in more than 150 clinical trials in the United States and around the world to treat conditions such as osteoarthritis, diabetes, emphysema and stroke.

The local Phase 1 trial has enrolled 16 of 24 total patients, and eight of them have completed the trial protocol, said Dr. Jeffrey Cohen, Cleveland Clinic neurologist and lead investigator of the trial.

So far, the treatment seems to be working, Cohen said.

“It’s a little early to be saying it, but things have looked encouraging.”

And there have been no safety concerns and almost no side effects. There has also been no activation — an aggravation or return of symptoms — of this relapsing disease in the patients involved, which has happened unexpectedly with other types of MS treatments.

Miller’s discovery won’t change the course of the trial currently under way at the Clinic and UH, but it may change the future of MSC treatment.

While they don’t know yet what the outcome of that trial will be, it’s possible that if a patient doesn’t respond to the treatment, it could mean that his stem cells aren’t producing enough HGF to be effective at healing, Miller said. Miller will be studying MSC samples from all the patients in the trial to find out if those who are better at producing HGF fare better.

He’ll also be trying to see if they can predict how well a patient will do based on his HGF levels in the MSC sample.

“Finally, though we’re a long way from this, maybe we could augment the expression of HGF in patients whose stem cells aren’t that effective to enhance their effectiveness,” he said.

But why not just inject the HGF alone? Miller said there are two reasons. First, the receptor for HGF in the cells, called c-MET, has been implicated in liver and breast cancer. Injecting HGF by itself into the body may stimulate the c-MET pathway, he said, and the research team is not willing to risk that.

“The stem cells have the advantage that they tend to home to the area of insult, so they don’t stick around in other parts of the body,” he said. “They target the treatment where it’s needed.”

Miller said his group is experimenting with a way of delivering HGF directly into the area of injury in the brain to minimize its contact with the rest of the body. HGF and c-MET are not associated with brain tumors.

They are also trying to test small fragments of the growth factor as a treatment, to see if they can eliminate some of the cancer concerns.

Cohen’s group hopes to have results from the Phase 1 trial available in the spring and has already started planning a larger study based on those results.

http://www.cleveland.com/healthfit/index.ssf/2012/09/cleveland_researchers_find_key.html

Can MS be treated with stem cells?

In ALL ARTICLES on October 25, 2011 at 2:13 pm

For more information and to see if you are a candidate for adult stem cell treatment: http://bit.ly/PATIENTQUESTIONNAIRE

I did some digging and would like to present the preliminary results to answer the question:

Can MS be treated with stem cells?

https://i2.wp.com/www.msconnections.org/ms-myelin.gifSOME MULTIPLE SCLEROSIS CLINICAL TRIALS

  • Hematopoietic stem cell transplantation for multiple sclerosis – 2002 clinical trial based on data collected from TWENTY medical institutes around the world.  Conclusion: Autologous HSCT suggest positive early results in the management of progressive MS and is feasible – http://www.springerlink.com/content/1b19ldgyecqvny3w/
  • Autologous Stem Cell Transplantation in Progressive Multiple Sclerosis, Sep 16, 1999  These results appear better than those achieved by any other treatment of progressive multiple sclerosis, including beta-interferon… – http://www.springerlink.com/index/H6X0866N90633266.pdf
  • “Dose of stem cells reverses some MS” – January 31, 2009 – STUDY – “A dose of their own stem cells “reset” the malfunctioning immune system of patients with early-stage multiple sclerosis and, for the first time, reversed their disability, according to researchers at Northwestern University in Chicago. Three years after being treated, on average, 17 of the 21 patients had improved on tests, suffering fewer problems with their balance or vision, 16 had experienced no relapse, and none had deteriorated.  This marks the first “reversal” of neurologic loss caused by this disease, says Richard Burt of Northwestern University in Chicago.
  • More – https://repairstemcell.wordpress.com/2009/09/02/multiple-sclerosis-and-stem-cells-need-more-info/

————————————-

Can MS be treated with stem cells?

You decide. Here are 11 articles from my blog,  28,000 from google news and there are a total of 20 clinical trials from clinicaltrials.gov

From my blog…

Autologous Stem Cell Transplants, Visible Progress in 8 of 10 Patients

***https://repairstemcell.wordpress.com/2010/05/18/ms-and-stem-cells-time-is-brain-in-ms/

*** https://repairstemcell.wordpress.com/2009/09/02/my-ms-pain-is-gone/

* * * https://repairstemcell.wordpress.com/2009/04/26/fat-derived-stem-cells-might-treat-ms-us-news-and-world-report/

* * * https://repairstemcell.wordpress.com/2009/04/15/britt%e2%80%99s-fight-against-multiple-sclerosis-hits-home-for-uncw-starnewsonlinecom-star-news-wilmington-nc/

* * * https://repairstemcell.wordpress.com/2009/04/09/stem-cell-breakthrough-may-lead-to-ms-treatments-forbescom/

* * * https://repairstemcell.wordpress.com/2009/04/07/my-battle-with-and-victory-over-ms-by-preston-walker-part-2/

* * * https://repairstemcell.wordpress.com/2009/04/07/my-battle-with-and-victory-over-ms-by-preston-walker-part-1/

* * * https://repairstemcell.wordpress.com/2009/03/07/study-stem-cell-transplants-could-reverse-ms-cloning-and-stem-cells-msnbccom/

* * * https://repairstemcell.wordpress.com/2009/02/23/foxnewscom-college-student-with-multiple-sclerosis-symptom-free-after-stem-cell-treatment-health-news-current-health-news-medical-news/

* * * https://repairstemcell.wordpress.com/2009/02/12/stem-cells-ms-multiple-sclerosis-cured-by-adult-stem-cells-video-stem-cell-research-and-stem-cell-therapy/

* * * https://repairstemcell.wordpress.com/2009/02/11/multiple-sclerosis-ms-stem-cell-trials-us-is-starting-to-%e2%80%9ccatch-up%e2%80%9d/

* * * https://repairstemcell.wordpress.com/2009/02/10/the-us-is-playing-catch-up-with-stem-cells-ms/

* * * https://repairstemcell.wordpress.com/2009/02/08/stem-cell-transplants-help-ms-victims/

…and about 28,000 scholarly papers/studys on “MS stem cell” here:
http://scholar.google.com/scholar?q=multiple%20sclerosis%20trial%20stem%20cell&oe=utf-8&rls=org.mozilla:en-US:official&client=firefox-a&um=1&ie=UTF-8&sa=N&hl=en&tab=ws\

…and 20 responses from clinicaltrials.gov on “multiple sclerosis stem cell”
http://clinicaltrial.gov/ct2/results?term=multiple+sclerosis+stem+cell

For more information and to see if you are a candidate for adult stem cell treatment: http://bit.ly/PATIENTQUESTIONNAIRE

Autologous Stem Cell Transplants, Visible Progress in 8 of 10 Patients

In STEM CELLS IN THE NEWS on October 25, 2011 at 1:49 pm

Transplanting stem cells from one’s own bone marrow (autologous stem cell transplants) improves the symptoms of muscular sclerosis (MS), and in some cases the neurological disease actually regressed. These are the encouraging results obtained from a small study performed on 21 remittent MS patients by a group from the Northwestern University School of Medicine in Chicago and published in Lancet Neurology. “All of the patients,” said the neurologists, “witnessed an improvement in their conditions three years after the stem cell transplants were performed. Of these, 81pct benefited from visible progress, measured in terms of the scale of their disability.”

With these “encouraging” results, the researchers decided to perform another study involving more patients, and compared their results with traditional treatments. The experiment involved the removal and freezing of bone marrow after treating the marrow with drugs to remove the lymphocytes of the immune system which are responsible for attacking nerve system fibers in MS patients.

At the end of the process, doctors implanted stem cells, which proved to be able to give the MS patients a normally functioning immune system. The time factor, underlined neurologists, seems crucial in the efficiency for stem cells because “if we intervene quickly, we are able to exploit the body’s ability to repair itself, which in the long term is lost”. In any case, scientists say that “ it is not a definitive cure for muscular sclerosis”.

Method weeds out best stem cells for MS

In STEM CELLS IN THE NEWS on October 21, 2011 at 9:18 am

“The scientists then injected the cells into the brains of mice that were born without the ability to make myelin. Twelve weeks later, the cells had become oligodendrocytes and had coated more than 40 percent of the brain’s neurons with myelin—a four-fold improvement over their earlier results.”

This is why stem cell treatments for MS work! – David

Method weeds out best stem cells for MS

U. BUFFALO/ U. ROCHESTER (US) — Scientists have discovered a precise way to isolate the specific stem cells needed to treat multiple sclerosis and a variety of childhood diseases caused by the brain’s inability to make myelin.

Twelve weeks after scientists injected specific stem cells into the brains of mice that were born without the ability to make myelin, the cells had coated more than 40 percent of the brain’s neurons with myelin (areas in red)—a four-fold improvement over earlier results. (Credit: University at Buffalo)

Myelin is the crucial fatty material that coats neurons and allows them to signal effectively. The research, reported online in Nature Biotechnology, overcomes an important barrier to using stem cells to treat demyelinating diseases.

Until now, it has been difficult to separate out the right progenitor cells—the ones that will develop into cells that make myelin—says Fraser Sim, assistant professor of pharmacology and toxicology at the University at Buffalo and the study’s first and co-corresponding author. He conducted most of the work as a researcher at the University of Rochester.

Straight from the Source:Read the original study – DOI: 10.1038/nbt.1972

…Sim and Rochester graduate student Crystal McClain ran extensive analyses looking at gene activity in different types of stem cells, leading to the conclusion that stem cells carrying a protein known as CD140a on their surface seemed to be most likely to become oligodendrocytes—the type of brain cell that makes myelin.

The scientists then injected the cells into the brains of mice that were born without the ability to make myelin. Twelve weeks later, the cells had become oligodendrocytes and had coated more than 40 percent of the brain’s neurons with myelin—a four-fold improvement over their earlier results.

…“These cells migrate more effectively throughout the brain, and they myelinate other cells more quickly and more efficiently than any other cells assessed thus far. Now we finally have a cell type that we think is safe and effective enough to propose for clinical trials.”
…The new approach may also be applicable to Krabbe’s Disease, Sim says, which also involves the breakdown of myelin. Sim, who came to UB in 2009, is actively collaborating on related work with researchers at the Hunter James Kelly Research Institute, a partnership between UB and the Hunter’s Hope Foundation and located in UB’s New York State Center of Excellence in Bioinformatics and Life Sciences.

The work was supported by the National Institute of Neurological Disorders and Stroke, the National Multiple Sclerosis Society, the New York State Stem Cell Research Board, the Adelson Medical Research Foundation and the Mathers Charitable Foundation.

NEW MS TREATMENT INFO!!!

In BEST OF THE BEST on October 6, 2011 at 1:02 am

NEW MULTIPLE SCLEROSIS TREATMENT INFO!!!!

For those interested, I just received 13 pages of Stem Cell Treatments of MS info. There is editing and translation and I am trying to get fully substantiated results for these treatments. I will have it to those of you who are interested shortly.

https://i0.wp.com/www.quicktrimbody.com/wp-content/uploads/2011/02/multiple-sclerosis-and-sunshine8.gif

Stem Cell Breakthrough May Lead to MS Treatments – Forbes.com

In ALL ARTICLES, STEM CELLS IN THE NEWS on April 9, 2009 at 4:58 pm

Now all they have to do is resolve the cancerous tumors issues that embryonic stem cells turn into….and address the associated transplant rejection that requires immunosuppressive drugs for life.  OR…they can go to any of the 5 stem cell treatment clinics around the world that use adult stem cells to make MS sufferers symptom free for years.  Are they once again missing the forest for the trees? Yes. -dg

ms-map-of-world1-stem-cell-myelin-sheeth

Map of MS prevalence throughout the world

Stem Cell Breakthrough May Lead to MS Treatments

04.08.09, 08:00 PM EDT

Efforts produce human cells that might someday help repair damaged nerves

THURSDAY, April 9 (HealthDay News) — U.S. scientists say they’ve coaxed human embryonic stem cells into generating cells that might someday be used to repair nerves damaged by multiple sclerosis.

The researchers pushed the stem cells to grow into critical nervous system support cells called oligodendrocytes, according to a report released Thursday.

Oligodendrocytes produce the myelin sheath that surrounds nerve fibers like wire insulation. The findings represent an important step toward embryonic stem cell-based therapies in general, experts say, and also for cell-based therapies for myelination disorders such as MS in particular. At the very least, the findings should lead to a laboratory model of the illness’ pathology.

“They are definitely laying the groundwork for being able to apply these cells in terms of a therapeutic application,” said Timothy Coetzee, executive director of Fast Forward, a wholly-owned subsidiary of the National Multiple Sclerosis Society, which partially funded the study.

Yet at the same time, he added, “It illustrated for me the critical importance of not assuming that because you can do something with a mouse cell, that a human cell is going to behave in the same manner.”

The research was published in t…

via Stem Cell Breakthrough May Lead to MS Treatments – Forbes.com.

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