DAVID GRANOVSKY

Posts Tagged ‘ipsc’

DO ADULT STEM CELLS CAUSE CANCER?

In ALL ARTICLES, SCIENCE & STEM CELLS, STEM CELLS IN THE NEWS, VICTORIES & SUCCESS STORIES on July 1, 2014 at 4:31 pm

NO!

cancer-free-zone

What do you get when you add 1,100 ADULT STEM CELL PATIENTS studied over 5 years,

plus another 1,873 ADULT STEM CELL PATIENTS studied over an average 12.5 years,

plus another ~7,000 ADULT STEM CELL PATIENTS in studies and FDA clinical trials data?

~10,000 ADULT STEM CELL TREATMENT PATIENTS showing NO CANCER!

 

“A total of 1873 patients were treated from 1990 to 2006 with bone marrow-derived concentrated cells. Patients were monitored for cancer incidence from the date of the first operation (1990) until death, or until December 31, 2011. The mean follow-up time was 12.5 years (range, five to twenty-two years)…No tumor formation was found at the treatment sites on the 7306 magnetic resonance images and 52,430 radiographs among the 1873 patients…This study found no increased cancer risk in patients after application of autologous cell-based therapy using bone marrow-derived stromal progenitor cells either at the treatment site or elsewhere in the patients after an average follow-up period of 12.5 years.

The upshot? This most recent study, as well as others, FDA clinical trials data, and the data we have published now amounts to about 10,000 patients who have been treated with adult stem cells and extensively tracked for this issue. There is no evidence that adult stem cells cause cancer. There is no rational reason for the fear, other than a completely different type of cell (ESC) that happens to have a similar name (stem cell) can cause teratomas – hence the confusion!”

Tumors, cancers and teratomas may result from Embryonic stem cell (ESC) treatments or Induced Pluripotent stem cell (iPSC) treatments

but NOT FROM ADULT STEM CELL TREATMENTS.

Sources:

A SIGHT FOR SORE EYES

In ALL ARTICLES, SCIENCE & STEM CELLS, STEM CELLS IN THE NEWS on June 28, 2014 at 1:45 pm

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A Sight for Sore Eyes
Author: Sarah Hoffman

“Researchers have grown part of an eye in a lab dish, using a type of stem cell made from a piece of skin.

They said the little retina started growing and developing on its own — an important step towards creating custom-tailored organs in the lab.”

Earlier this month scientists successfully created a functioning human retina using iPSCs (induced pluripotent stem cells). But that’s not the cool part. What’s actually amazing is that they did very little to make this happen.

Wait what?! Ok let me explain. These researchers took some of these cells from a tiny little piece of human skin, basically rewound time as far as these cells are concerned and pushed them back to a more-or-less embryonic state, sent signals to some genes manipulating them to form a retina, and then let it do it’s thing. And it was a success! This little retina is living in a dish just sensing light and being a badass little organ. Even the scientists didn’t realize this would happen so naturally

Autism recreated from stem cells in lab study

In VICTORIES & SUCCESS STORIES on February 14, 2011 at 3:54 pm

INDUCED PLURIPOTENT STEM CELLS (IPSC) are not the stem cells we usually hear about because they are human skin cells (typically) that are regressed to an embryonic state.  The problem with the is that they carry the genetic abnormalities of the donor and they form cysts and tumors like embryonic stem cells.  SO they are useless for treating diseases until these problems are resolved.  Or are they?  Scientists have used IPSC to grow cells with Autism.  They can now follow the path of the disease  almost from embryo to fully mature cells and will learn a lot more about it. – dg

autism-ribbon

“Their findings, published in the Nov. 12, 2010, issue of Cell, revealed disease-specific cellular defects, such as fewer functional connections between Rett neurons, and demonstrated that these symptoms are reversible, raising the hope that, one day, autism maybe turn into a treatable condition.

“Mental disease and particularly autism still carry the stigma of bad parenting,” says lead author Alysson Muotri, Ph.D., an assistant professor in the Department of Molecular and Cellular Medicine at the University of California, San Diego School of Medicine.

“We show very clearly that autism is a biological disease that is caused by a developmental defect directly affecting brain cells.”

Stem cell research news: Autism recreated from stem cells in lab study – Gilbert Special Needs Kids | Examiner.com.

Stem-cell furore erupts : Nature News

In STEM CELLS IN THE NEWS on June 29, 2010 at 2:21 pm

What are iPSc?  iPSc are INDUCED PLURIPOTENT STEM CELLS.  In short, a scientist takes a skin cell, typically from the foreskin or testes, and regresses or devolves them into an embryonic-like stem cell (sort of like a test tube version of Benjamin Button).

It was originally hoped that IPSc would hold great promise because they had all of the benefits of embryonic stem cells (read – Pluripotency: the ability to differentiate into any of the 320+ cells in the human body) and none of the drawbacks.  Here is what we know about Embryonic stem cells:

  1. Embryonic stem cells form Cysts/Tumors
  2. Embryonic stem cells are associated with tons of Controversy
  3. After 10 years, no treatments have been developed from embryonic stem cells
  4. Many of the foremost Embryonic stem cell scientists have abandoned embryonic research for adult stem cell and iPSc research

Now, here’s the kicker.  After 2 years, extensive research based on the originally ground breaking article and a huge media following (over 4,000,000 hits on google), it turns out that not only DO iPSc form cysts/tumors…but they also may NOT be pluripotent at all.

Why did it take so long?  “Skutella (the author of the original paper)  and his co-authors said that they wanted to share the cells but that the original agreement signed by tissue donors precluded distribution to third parties….even though Nature requires its authors to share all published research resources”

Regardless of how this turns out, I guess iPSc are like embryonic stem cells:

  1. iPSc form Cysts/Tumors (like embryonic stem cells)
  2. iPSc are NOW associated with tons of Controversy (like embryonic stem cells)
  3. After 2 years, no treatments have been developed from iPSc (like embryonic stem cells)

Is this a witch hunt on Skutella? Is this a fraud perpetrated by him? Is this just a misunderstanding?  It remains to be seen but as a pivitol paper that has launched (or may end) an entire field of research and commercial treatment potential, a certain degree of data transparency should be expected.

Since when does scientific research have more drama than Grey’s Anatomy? Keep your “Eyes on the Ball” guys, “Eyes on the Ball!”  -dg

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Stem-cell furore erupts

Data analysis ignites public row.

Published online 29 June 2010 | Nature | doi:10.1038/466017a – Alison Abbott

Thomas Skutella.

Long-rumbling hostilities between stem-cell researchers in Germany exploded into a blazing public row last week, after Nature published a critical reanalysis of data from a high-profile 2008 article.

The researchers behind the original work1, led by Thomas Skutella of the University of Tübingen, reported using cells from adult human testes to create pluripotent stem cells with similar properties to embryonic stem cells.

Unlike other adult cells, these reproductive or ‘germline’ stem cells can be reprogrammed for pluripotency without the need to introduce additional genes, a step that often relies on a virus. That could make them safer for future use in medicine.

The paper made headlines because such pluripotent stem cells might be used instead of ethically sensitive human embryonic tissue. Soon after its publication, however, some stem-cell scientists said that the evidence for pluripotency was unconvincing. They also complained that Skutella would not distribute cells to other labs for verification, even though Nature requires its authors to share all published research resources.

Hans Schöler, a director at the Max Planck Institute for Molecular Biomedicine in Münster and an author of last week’s critical comment2, says that he proclaimed Skutella’s achievement as a breakthrough when he first saw the data at a meeting, but became doubtful after seeing the published paper. “If this paper is wrong, then a lot of scientists are wasting time, energy and money in trying to follow up on it,” he says. Others fear that the episode is undermining the credibility of the field.

In response, Skutella last week asked the DFG, Germany’s main research-funding agency, to conduct an investigation both of his paper in Nature and of what he claims is a witch-hunt against him. Schöler, who also works with germline stem cells, says that he would welcome such a move.

Pluripotent cells should form teratomas (often cancerous cysts) — encapsulated tumours comprising different cell types — when injected under the skin of mice, and also exhibit a particular profile of gene expression. “The teratoma pictures in the Nature paper were not terribly convincing but that didn’t concern me too much at first,” says Schöler. “It was the failure to provide cells that started to concern me.” After more than a year of requests for access, he decided to reanalyse data in the paper in Nature showing which genes in the disputed cells were being expressed.

Together with bioinformaticians, he compared the genes’ expression profile with those of other cells in public databases and found that it overlapped with a type of connective-tissue cell called fibroblasts but not with pluripotent stem cells. Schöler suggests that fibroblasts may have contaminated Skutella’s samples. But Skutella and his colleagues deny3 mistaking fibroblasts for pluripotent cells. Skutella says that comparison of gene-expression data is meaningless “if the cells being compared were not processed identically”.

Takashi Shinohara at Kyoto University in Japan, whose team in 2004 generated the first pluripotent germline stem cells from mice, shares Schöler’s concerns about the expression data. He says that fibroblasts and pluripotent cells have different gene-expression profiles even if the cells are not processed in similar ways, and adds that it would be helpful to see Skutella’s cells.

In a corrigendum to his original paper in August 2009, Skutella and his co-authors said that they wanted to share the cells but that the original agreement signed by tissue donors precluded distribution to third parties. (Really???) Having gained broader consent from some donors, Skutella now promises to distribute the cells once they have been quality-checked. But stem-cell researcher Rudolf Jaenisch at the Whitehead Institute for Biomedical Research in Cambridge, Massachusetts, is not impressed: “It’s a big problem not providing the cells for what is nearly two years — whatever the excuses, this is bad.”

Ulrike Beisiegel, ombudsman for the DFG, says her office will decide “soon” whether to take up the investigations.

via Stem-cell furore erupts : Nature News.

Cellular Dynamics’ stem cells named to top 10 emerging technologies list – JSOnline

In BUSINESS OF STEM CELLS on April 21, 2010 at 5:07 pm

A few things of note:
1.  This article is about James Thomson putting his money where his mouth is…into non-embryonic stem cell research.  Long ago, he jumped off the embryonic stem cell research “ship” and into other stem cell research.  This is fairly significant because he is the one who built that ship from the ground up!  (
James Alexander Thomson is an American developmental biologist who is best known for deriving the first human embryonic stem cell line.)

2.  This article is about induced pluripotent stem cells: “The stem cells (Cellular Dynamics) CDI sells aren’t made from human embryos. CDI scientists take tissue cells, for example, and engineer them to make cells that have the characteristics of embryonic stem cells and are able to turn into beating heart cells, liver cells or other cells in the body.”  Further of note, adult or repair stem cells are already “able to turn into beating heart cells, liver cells or other cells in the body and have been for years.”

3.  Those guys over at MIT Technology Review are pretty smart.  They know the future is NOT in embryonic stem cell research…just as James does…or more importantly, they know that embryonic stem cells are NOT one of the “top 10 emerging technologies.”  Otherwise, something with embryonic stem cells would have made the list.  Or maybe Geron would have with their embryonic clinical trial that has been put on hold 11 times now…what?  It’s 12 or 13? Sorry, I can’t keep track anymore.

Read on…

Cellular Dynamics’ stem cells named to

top 10 emerging technologies list

By Kathleen Gallagher of the Journal Sentinel

Posted: April 21, 2010 11:54 a.m. |(2) Comments

James Thomson’s engineered stem cells have joined green concrete, mobile 3-D content and other innovations on the MIT Technology Review’s annual list of its top 10 emerging technologies.

Stem cell pioneer Thomson and his company, Cellular Dynamics, have potentially revolutionized the way disease is studied and potential drugs are screened for toxicity, the editors of the publication said.

The privately held company, known as CDI, has a proprietary process to manufacture large quantities of human tissue cells that companies use for research and to screen drugs.

“Stem cell technology has significant potential to improve human health,” Thomson said in a news release issued by his company.

The stem cells CDI sells aren’t made from human embryos. CDI scientists take tissue cells, for example, and engineer them to make cells that have the characteristics of embryonic stem cells and are able to turn into beating heart cells, liver cells or other cells in the body.

via Cellular Dynamics’ stem cells named to top 10 emerging technologies list – JSOnline.

STEM CELL VICTORIES AND MEDICAL FAILURES

In STEM CELLS IN THE NEWS on March 5, 2010 at 12:50 pm
FAILURES + VICTORIES

For years, precious few reliable resources have pointed out the failures of Embryonic Stem Cell (ESC) research for treatments and the dishonest practices within the US medical system while also bringing you the latest Repair Stem Cells (RSC) victories.  Misinformation and confusion on these issues is often exacerbated by the unequal media coverage in the USA.  Virtually every news source pushes unproven Embryonic hype while refusing to report the huge number of Repair Stem Cell clinical trials, study results and real life stories.  A virtual wall has existed, obscuring the truth and separating reality from fantasy and perpetuated by fiction, ignorance and misinformation.

The tide though, is starting to turn!

Story after story of Embryonic and medical system failures are all over the press recently and the unrelenting flow of Repair Stem Cell victories are now also starting to nudge their way onto the world stage (including in the USA)!

NIH Director Promotes Politics Over Adult Stem Cell Research, Sound Science

In CATCH UP! on January 7, 2010 at 3:17 pm

NIH Director Promotes Politics Over Adult Stem Cell Research, Sound Science

by Steven Ertelt, LifeNews.com Editor, January 4, 2010

Washington, DC (LifeNews.com) — In a new opinion column appearing the magazine Science, NIH director Francis Collins appears to put politics over science. In a list of five “opportunities for research,” Collins promotes embryonic stem cell research but ignores the adult variety already helping patients.

In the column, Collins says it is “appropriate to identify areas of particular promise” where NIH can help fund the work of scientists and researchers in “areas that are ripe for major advances that could reap substantial downstream benefits.”

“Yet when discussing stem cell research and Translational Medicine, there is no mention whatsoever of pushing ahead with developing more adult stem cell treatments, already shown effective for patients,” notes Family Research Council fellow Dr. David Prentice. “Instead, the emphasis is all on embryonic stem cells and the embryonic-like iPS cells.”

Collins promotes the first human protocol (for spinal cord injury) involving human embryonic stem cells and says it was approved by the FDA in 2009. The NIH director says “the opening up of federal support for hESC research will bring many investigators into this field.” Collins also spends time touting iPSCs even while admitting “much work remains to be done to investigate possible risks.”

While pro-life advocates share the excitement about induced pluripotent stem cells (iPSCs), they note that significant hurdles have not been overcome that would bring it to the level of adult stem cells — that are helping patients today battle more than 100 different diseases and condition.

Prentice, a former Indiana State University biology professor, says “Collins continues to pump the [Obama] Administration line on stem cells, emphasizing embryonic stem cells and completely ignoring the only stem cells helping patients now — adult stem cells.”

“This is not the first time Collins has seemed ignorant of adult stem cell successes. But it is still disappointing to see the emphasis on political science instead of putting the patients first,” Prentice says.

In a previous interview with the New England Journal of Medicine, Francis Collins talks about the number of “stem cell” clinical trials. The “one clinical trial” Collins refers to is the one embryonic stem cell experiment with patients that is out there. And it is indeed on hold. But there are at least 2,000 clinical trials for Adult Stem Cells,” Prentice says. “By the way, there are quite a few done on the NIH campus itself.”

via NIH Director Promotes Politics Over Adult Stem Cell Research, Sound Science.

University of Edinburgh study paves way for stem cell library » The Journal

In VICTORIES & SUCCESS STORIES on October 29, 2009 at 2:15 am

University of Edinburgh study paves way for stem cell library

https://i0.wp.com/www.hepfoundation.org.nz/images/liver2.jpg

Research could revolutionise the development of drugs to treat diseases and pave way for the creation of a library of liver cells

by Chris Grainger –  Wednesday 28 October 2009, The Journal Issue 26

Scientists have, for the first time, produced liver cells from adult stem cells using technology called iPSC, or induced pluripotent stem cell.

Using either embryonic or induced pluripotent stem cells, the Gamm lab created aggregates of early retinal cells (green spheres). The blue spheres are early brain cells. Photo: Special arrangement - University of Wisconsin

Using induced pluripotent stem cells, aggregates of early retinal cells (green spheres) are created. The blue spheres are early brain cells. Photo: Special arrangement - University of Wisconsin

The liver cells were created by manipulating the skin cells to resemble embryonic stem cells, which have the ability to become different cells within the body.

The study, led by the University of Edinburgh’s Medical Research Council Centre for Regenerative Medicine, makes possible the creation of a liver cell library, which could revolutionise the development of drugs, making them more efficient and safe.

https://i1.wp.com/www.meritas.net/uploaded/school_graphics/Edinburgh_University.jpg

University of Edinburgh

via University of Edinburgh study paves way for stem cell library » The Journal.

Stem Cells for Newbies ;)

In ALL ARTICLES, STEM CELLS - 101 on April 18, 2009 at 10:26 am

S0metimes we get so involved with what we are doing, we forget that there are people who are just tuning in now with no frame of reference for what the hell we are talking about.  SO here it is…Stem Cells for Newbies!

I'm a stem cell newbie!

I'm a stem cell newbie!

The world of stem cells is changing all the time but the big 3 are:

ESC = embryonic stem cell
•can currently treat zero diseases
•lotsa problems (tumors, rejection, controversy, etc)
•comes from embryos

ASC = adult stem cell
•used in bone marrow transplants for 40 years
•can currently treat 130+ diseases
•~zero problems (virtually zero side effects)
•comes from blood, umbilical cords, marrow, fat, nose, breast milk, menstruation, etc etc etc

iPSC = induced pluripotent stem cell

•fairly new
•lotsa potential (needs more research)
•may be the best of both worlds
•comes from regular skin cells that are then magically transformed by scientists into stem cells

Now…if you have any follow up questions, feel free to ask.  You have been fed info about stem cells for 6-8 years that has been mostly incorrect so no question is stupid.  Ask away!

Regards-

David

Former Vice President Gore Jumps Into Stem Cell Research

In ALL ARTICLES, STEM CELLS IN THE NEWS on April 14, 2009 at 6:01 pm

gore-ips-cells

Former Vice President Gore Jumps Into Stem Cell Research

Tuesday, Apr 14, 2009 @09:30am CST

Former Vice President Al Gore is interested in more than global warming issues.

Today he’ll put his name behind a 20-million dollar venture involving stem cell research.

“USA Today” says the program will involve an alternative to embryonic stem cells called “induced pluripotent” stem cells.

Mr. Gore says the cells are “filled with promise and hope.” He says, quote, “I think this is one of those good news stories that comes along every once in a while.”

When he was a member of Congress in the 1970s and 80s, Gore was involved in hearings on medical uses of cell technologies.

Now he’s a partner with a venture-capital firm, Kleiner Perkins Caufield and Byers.

That group will work with researcher Shinya Yamanaka of Kyoto University.

The scientist announced three years ago he’d developed a way to reprogram skin cells, creating something close enough to an embryonic cell that it has potential to grow transplantable organs.

The first efforts will zero in on cells with genetic markers for Parkinson’s and a couple of lesser known diseases in hopes of finding a cure.

via Former Vice President Gore Jumps Into Stem Cell Research.

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