DAVID GRANOVSKY

Posts Tagged ‘embryo’

WHAT WILL TOM PRICE MEAN TO PATIENTS?

In BUSINESS OF STEM CELLS, HEALTH AND WELLNESS, STEM CELLS IN THE NEWS on January 24, 2017 at 11:45 am

tom_price_official_transition_portrait

“Who is Tom Price, Trump’s pick for HHS?

Washington (CNN) While some Republicans have signaled major changes to Obamacare are a long time in the making, President-elect Donald Trump has sent a strong signal that the law’s days are numbered, no matter what.

That signal is Rep. Tom Price, Trump’s pick to head the Department of Health and Human Services.
The Georgia Republican and medical doctor is among the law’s most studied and determined opponents.” via

On ABORTION

  • Voted YES on banning federal health coverage that includes abortion. (May 2011)
  • Voted NO on expanding research to more embryonic stem cell lines. (Jan 2007)
  • Voted NO on allowing human embryonic stem cell research. (May 2005)
  • Voted YES on restricting interstate transport of minors to get abortions. (Apr 2005)
  • Rated 100% by the NRLC, indicating a pro-life stance. (Dec 2006)
  • Bar funding for abortion under federal Obamacare plans. (Jul 2010)
  • Prohibit federal funding for abortion. (May 2011)
  • Prohibit federal funding to groups like Planned Parenthood. (Jan 2011)
  • No family planning assistance that includes abortion. (Jan 2013)
  • Grant the pre-born equal protection under 14th Amendment. (Jan 2007)

On DRUGS

  • Voted NO on more funding for Mexico to fight drugs. (Jun 2008)
  • Rated -10 by NORML, indicating a “hard-on-drugs” stance. (Dec 2006)
  • Rated 0% by NORML, indicating an anti-legalization stance. (Jan 2014)

On ENVIRONMENT

  • Voted NO on $2 billion more for Cash for Clunkers program. (Jul 2009)
  • Voted NO on protecting free-roaming horses and burros. (Jul 2009)
  • Voted NO on environmental education grants for outdoor experiences. (Sep 2008)
  • Voted NO on $9.7B for Amtrak improvements and operation thru 2013. (Jun 2008)
  • Voted NO on increasing AMTRAK funding by adding $214M to $900M. (Jun 2006)
  • Voted NO on barring website promoting Yucca Mountain nuclear waste dump. (May 2006)
  • Voted YES on deauthorizing “critical habitat” for endangered species. (Sep 2005)
  • Stop considering manure as pollutant or hazardous. (Sep 2011)
  • Rated 13% by HSLF, indicating an anti-animal welfare voting record. (Jan 2012)
  • Strengthen prohibitions against animal fighting. (Jan 2007)

On HEALTH CARE

  • Republicans have offered ideas and solutions on healthcare. (Jan 2010)
  • Address lawsuit abuse; it doesn’t raise taxes by a penny. (Jan 2010)
  • More Medical Savings Accounts; less medical malpractice. (Nov 2004)
  • Voted YES on the Ryan Budget: Medicare choice, tax & spending cuts. (Apr 2011)
  • Voted YES on repealing the “Prevention and Public Health” slush fund. (Apr 2011)
  • Voted NO on regulating tobacco as a drug. (Apr 2009)
  • Voted NO on expanding the Children’s Health Insurance Program. (Jan 2009)
  • Voted YES on overriding veto on expansion of Medicare. (Jul 2008)
  • Voted NO on giving mental health full equity with physical health. (Mar 2008)
  • Voted NO on Veto override: Extend SCHIP to cover 6M more kids. (Jan 2008)
  • Voted NO on adding 2 to 4 million children to SCHIP eligibility. (Oct 2007)
  • Voted NO on requiring negotiated Rx prices for Medicare part D. (Jan 2007)
  • Voted YES on denying non-emergency treatment for lack of Medicare co-pay. (Feb 2006)
  • Repeal any federal health care takeover. (Jul 2010)
  • Deauthorize funding for Obamacare. (Jul 2010)
  • Repeal the Job-Killing Health Care Law. (Jan 2011)

Via 

 

On HEALTH ISSUES
Benjamin says that Price’s record in public-health policy is particularly worrying. In 2008, for instance, Price voted against allowing the FDA to regulate tobacco as a drug.

Price has also pushed to repeal the Public Health and Prevention Fund (PHPF), a roughly $1 billion to $2 billion fund provided yearly to the CDC to support public-health programmes. Owing to past budget cuts, the agency has used this money to bolster spending on existing programmes, such as research on lead toxicity. Price has criticized the PHPF as a “slush fund”, but Benjamin says that it has been essential for the agency. “Should he be confirmed, we’d be working very hard to try and change his mind to convince him that prevention is an important issue he should champion,” he says.

And Price has also consistently opposed embryonic stem cell research, saying in 2009 that Obama’s executive order to permit such research would “force taxpayers to subsidize research that will destroy human embryos”.

He has also supported numerous efforts to defund the reproductive non-profit healthcare group Planned Parenthood” Via

 

“On STEM CELL RESEARCH

Price has been outspoken against research that involves embryonic stem cells.
In 2005, Price spoke with Georgia’s Athens Banner-Herald newspaper about the “ethical dilemma” of stem cell research. Embryonic stem cells, which have the potential to treat myriad medical conditions, are controversial because they derive from early embryos.
The cells are of interest for research because they have the potential to develop into many different cell types, and so scientists believe they can be used to generate cells and tissues that could be used for cell-based therapies.

See the latest news and share your comments with CNN Health on Facebook and Twitter.

“There are people who believe any form of embryonic stem cell research necessitates the destruction of human life,” Price said. “I can’t overestimate the importance of that statement.” And for people who believe that, “stem cell scientists are threatening your fundamental principles,” he said.
According to the National Institutes of Health’s current guidelines on human stem cell research, embryonic stem cells are eligible for research with NIH funding if they were created using in vitro fertilization and are no longer needed or were donated by individuals seeking reproductive treatment.
Price has voted against expanding embryonic stem cell research. As HHS secretary he would oversee NIH grants to research on embryonic stem cells.” Via
On LGBT, DOMESTIC VIOLENCE, HUMAN RIGHTS, PERSONHOOD STATUS of EMBRYOS
Last year Price joined other Georgia Congressmen in signing a letter of support for fired Atlanta fire chief Kelvin Cochran, who was terminated after not obtaining permission to use his official capacity to promote a book he wrote that disparages LGBT people under the cloak of religion. The letter falsely described both Cochran’s actions and the reason for his termination.Price, as On The Issues details, voted against reauthorizing the Violence Against Women Act and against prohibiting job discrimination based on sexual orientation. He voted to constitutionally define marriage as one-man-one-woman and to amend the Constitution to define traditional marriage. He’s earned a 0% rating from the Human Rights Campaign and a 17% rating from the NAACP.

Rep. Price, 62, opposes a woman’s right to choose, he opposes stem cell research, and supports banning all federal funding of Planned Parenthood. He also supports granting embryos personhood status and thus equal protection under 14th Amendment.” Via 

 

What will Tom Price as head of the Department of Health and Human Services mean to you?  Please comment below.

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DO ADULT STEM CELLS CAUSE CANCER?

In ALL ARTICLES, SCIENCE & STEM CELLS, STEM CELLS IN THE NEWS, VICTORIES & SUCCESS STORIES on July 1, 2014 at 4:31 pm

NO!

cancer-free-zone

What do you get when you add 1,100 ADULT STEM CELL PATIENTS studied over 5 years,

plus another 1,873 ADULT STEM CELL PATIENTS studied over an average 12.5 years,

plus another ~7,000 ADULT STEM CELL PATIENTS in studies and FDA clinical trials data?

~10,000 ADULT STEM CELL TREATMENT PATIENTS showing NO CANCER!

 

“A total of 1873 patients were treated from 1990 to 2006 with bone marrow-derived concentrated cells. Patients were monitored for cancer incidence from the date of the first operation (1990) until death, or until December 31, 2011. The mean follow-up time was 12.5 years (range, five to twenty-two years)…No tumor formation was found at the treatment sites on the 7306 magnetic resonance images and 52,430 radiographs among the 1873 patients…This study found no increased cancer risk in patients after application of autologous cell-based therapy using bone marrow-derived stromal progenitor cells either at the treatment site or elsewhere in the patients after an average follow-up period of 12.5 years.

The upshot? This most recent study, as well as others, FDA clinical trials data, and the data we have published now amounts to about 10,000 patients who have been treated with adult stem cells and extensively tracked for this issue. There is no evidence that adult stem cells cause cancer. There is no rational reason for the fear, other than a completely different type of cell (ESC) that happens to have a similar name (stem cell) can cause teratomas – hence the confusion!”

Tumors, cancers and teratomas may result from Embryonic stem cell (ESC) treatments or Induced Pluripotent stem cell (iPSC) treatments

but NOT FROM ADULT STEM CELL TREATMENTS.

Sources:

ROUND UP DAMAGES EMBRYONIC, PLACENTAL AND UMBILICAL CORD CELLS

In ALL ARTICLES, OFF THE BEATEN PATH, STEM CELLS IN THE NEWS on June 20, 2014 at 12:23 pm

roundup-the-ultimate-killing-machine

ROUND UP DAMAGES EMBRYONIC, PLACENTAL AND UMBILICAL CORD CELLS

Chemicals in Round Up are targeting both our reproductive abilities and our regenerative/healing abilities.  But we’ve know this for a long time.  What’s special about this article is that it is in Scientific American, “The leading source and authority for science, technology information and policy for a general audience”

From the article:
“Monsanto scientists argue that cells in Seralini’s study were exposed to unnaturally high levels of the chemicals…Seralini’s team, however, did study multiple concentrations of Roundup. These ranged from the typical agricultural or lawn dose down to concentrations 100,000 times more dilute than the products sold on shelves. The researchers saw cell damage at all concentrations.”

So while Monsanto scientists say the study used unnaturally high amounts, this is false,  The study included samples 100,000 TIMES MORE DILUTE than average exposures AND IT STILL CAUSED CELL DAMAGE.

Cell damage to

  • EMBRYONIC CELLS
  • PLACENTAL CELLS
  • UMBILICAL CORD CELLS

The corner stone to both our reproductive and regenerative capabilities.  Think you’re safe? Roundup Weedkiller Found In 75% of Air and Rain Samples, Gov. Study Finds

You idiots.  You’ve killed us all.

statue_planet

http://www.scientificamerican.com/article/weed-whacking-herbicide-p/

STEM CELLS 101 – short

In ALL ARTICLES, SCIENCE & STEM CELLS on April 11, 2013 at 12:50 am

Transplanted adult dermal stem cells / Cellule...

STEM CELLS 101 – short

ADULT STEM CELL = ASC

  • SOURCE/DERIVED FROM•comes from
    blood, umbilical cords, bone marrow, placenta fat tissue, muscle, nasal
    neurological, breast milk, menstruation, dental pulp, lungs (new source!) and many more
  • PURPOSE IN BODY•they are the body’s natural healing cells
  • OBSTACLES+SIDE EFFECTS•~zero problems (virtually zero side effects)
  • TREATMENT HISTORY•used in bone marrow transplants to treat cancer for 40 years
  • TREATMENT HISTORY•can currently treat 130+ diseases safely and effectively (CP, MS, Autism, Diabetes, CHF, PAD, etc)

EMBRYONIC STEM CELL = ESC

  • SOURCE/DERIVED FROM•comes from embryos
  • PURPOSE IN BODY•split for 7 weeks until you have a fetus the size of a thumbnail
  • OBSTACLES+SIDE EFFECTS•they create
    cysts and tumors, rejection requires immunosuppressive drugs for the ill
    patient, they carry the genetic anomalies of the donor, etc
  • TREATMENT HISTORY•can currently treat zero diseases, probably need to cure cancer first to use them

INDUCED PLURIPOTENT STEM CELL = iPSC (“Embryonic Stem Cell Lite”)

  • SOURCE/DERIVED FROM•comes from regular adult cells like skin cells that are then transformed by scientists into stem cells
  • PURPOSE IN BODY•to be a skin cell or other tissue
  • OBSTACLES+SIDE EFFECTS•they create
    cysts and tumors, rejection requires immunosuppressive drugs for the ill
    patient, they carry the genetic anomalies of the donor…
  • TREATMENT HISTORY•no treatments to date, probably need to cure cancer first to use them

ORIGINS OF SCHIZOPHRENIA IDENTIFIED THROUGH STEM CELL RESEARCH

In ALL ARTICLES, SCIENCE & STEM CELLS, STEM CELLS IN THE NEWS on January 24, 2013 at 9:00 am

7schizophrenia2

Stem Cell Research Helps to Identify Origins of Schizophrenia

Jan. 22, 2013 — New University at Buffalo research demonstrates how defects in an important neurological pathway in early development may be responsible for the onset of schizophrenia later in life.  The UB findings, published in Schizophrenia Research, test the hypothesis in a new mouse model of schizophrenia that demonstrates how gestational brain changes cause behavioral problems later in life — just like the human disease.

The genomic pathway, called the Integrative Nuclear FGFR 1 Signaling (INFS), is a central intersection point for multiple pathways of as many as 160 different genes believed to be involved in the disorder. “We believe this is the first model that explains schizophrenia from genes to development to brain structure and finally to behavior,” says lead author Michal Stachowiak, PhD, professor in the Department of Pathology and Anatomical Sciences in the UB School of Medicine and Biomedical Sciences. He also is director of the Stem Cell Engraftment & In Vivo Analysis Facility at the Western New York Stem Cell Culture and Analysis Center at UB.

“A key challenge with the disease is that patients with schizophrenia exhibit mutations in different genes, he says.   How is it possible to have 100 patients with schizophrenia and each one has a different genetic mutation that causes the disorder?” asks Stachowiak. “It’s possible because INFS integrates diverse neurological signals that control the development of embryonic stem cell and neural progenitor cells, and links pathways involving schizophrenia-linked genes.  INFS functions like the conductor of an orchestra,” explains Stachowiak. “It doesn’t matter which musician is playing the wrong note, it brings down the conductor and the whole orchestra. With INFS, we propose that when there is an alteration or mutation in a single schizophrenia-linked gene, the INFS system that controls development of the whole brain becomes un-tuned. That’s how schizophrenia develops.”

“Using embryonic stem cells, Stachowiak and colleagues at UB and other institutions found that some of the genes implicated in schizophrenia bind the FGFR1 (fibroblast growth factor receptor) protein, which in turn, has a cascading effect on the entire INFS.  “We believe that FGFR1 is the conductor that physically interacts with all genes that affect schizophrenia.  We think that schizophrenia occurs when there is a malfunction in the transition from stem cell to neuron, particularly with dopamine neurons”, said Stachowiak. The researchers tested their hypothesis by creating an FGFR1 mutation in mice, which produced the hallmarks of the human disease: altered brain anatomy, behavioral impacts and overloaded sensory processes.

“By attacking the INFS pathway, we were able to produce schizophrenia in mice.”  He adds that if such a generalized genomic pathway is causing the disease, then it should be possible to treat the disease with a more generalized approach. “We may even be able to devise ways to arrest development of the disease before it presents fully in adolescence or adulthood.”

http://www.buffalo.edu/news/releases/2013/01.html

For More articles on Schizophrenia, Click HERE

WHAT IS SCHIZOPHRENIA????

“Psychological disorder is a mental disorder that impacts on life and creates fake experiences. There are many kinds of psychological disorder such as anxiety, childhood, eating, and gender identity disorder. Schizophrenia is one of childhood disorder that is occurred where people have difficulty to distinguish their reality from unreal and fake experiences.” – http://dasolkimuou.wordpress.com/2012/11/21/what-is-schizophrenia/

DISCOVERY OF PRIMARY CILIA IN STEM CELLS

In ALL ARTICLES, SCIENCE & STEM CELLS on January 7, 2013 at 10:40 am
“…since stem cells are now being more routinely used for regenerative medicine such as repair of severed spinal cord (Lu et al. 2012), it behooves us to better learn the molecular mechanisms that keeps these invaluable cells in an undifferentiated state so that we can harness their full therapeutic potential.”
Discovery of Primary Cilia in Stem Cells

Author: Aashir Awan, PhD

The primary cilium is organelle that has garnered much attention in the field of cell biology during the last 15 years. It is a slender, solitary hair-like organelle that extends 5-10 uM from each mammalian cell (in the G0 cell cycle state) that is microtubule-based (9 outer doublets arranged in a circular fashion) and dependent on a process called Intraflagellar Transport (IFT). IFT is the bidirectional movement of motors (kinesin-2 in the anterograde and dynein-2 in the retrograde direction) responsible for the assembly and maintenance of the cilium (Pedersen et al., 2006).

Until this time, it had been labeled a ‘vestigial’ organelle not worthy of research. Yet, a breakthrough into the sensory role of the primary cilium came in 2000 based on Dr. Rosenbaum’s research on Chlamydomonas and the motile cilium or flagella. Along with Dr. George Whitman’s group, they were able to show the importance of Tg737 (IFT88) protein to the pathology of polycystic kidney disease in mouse (Pazour et al., 2000). Since then, research into the primary cilium has exploded and has been linked to diverse pathologies (collectively known as ciliopathies) such as

  • retinitis pigmentosa,
  • hydrocephaly,
  • situs inversus,
  • ovarian and pancreatic cancers among others (Nielsen et al., 2008; Edberg et al., 2012). Also, various
  • signal transduction pathways have been found to be coordinated by the primary cilia such as hedgehog, wnt, PDGF among others (Veland et al., 2008).

Thus, in 2006, the Christensen lab at the University of Copenhagen (Denmark) with the collaboration of Dr. Peter Satir’s group at Albert Einstein College of Medicine (Bronx, NY) began to investigate whether the human embryonic stem cells (hESCs) possess primary cilium and then to begin preliminary molecular dissections of the role that this organelle could play in the proliferation and differentiation profiles of these pluripotent cells. The Albert Einstein group, due to NIH restrictions, had to work with two federally-sanctioned cell lines. Working with the Laboratory of Reproductive Biology at RigsHospital, the Danish side had access to in-house derived stem cell lines from discarded blastocysts. The advantage for the Danish side was obvious since these newer cell lines hadn’t undergone as many passages as the NIH cell lines and were thus more robust. To begin preliminary characterizations of these lines, some basic hallmarks of hESCs (Bernhardt et al., 2012) had to be localized to the nucleus such as the transcription factor (TF) Oct4 (Fig. 1).

In addition, a single primary cilium can be seen denoted by the acetylated tubulin staining emanating from each cell in the micrographs. Also, the base of the cilium is marked by the presence of pericentrin and centrin which demarcate the centriole.

Fig1 Fig. 1 Primary cilia stained with anti-acetylated tubulin (tb, red) are indicated by arrows and undifferentiated stem cells are identified by nuclear colocalization of OCT-4 (green) and DAPI (dark blue) in the merged image (light blue). A primary cilium (tb, red, arrow) in undifferentiated hESCs emerges from one of the centrioles (asterisks) marked with anti-centrin (centrin, green). Inset shows anti-pericentrin localization to base of cilia (Pctn, green).

Together, the three labs were the first to discover primary cilia in stem cells while other groups have since then confirmed these findings (Kiprilov et  al. 2008; Han et al. 2008). Attention was then to characterize different signal transduction pathways in the stem cell cilium. Since the hedgehog pathway has been shown to be important for differentiation and proliferation (Cerdan and Bhatia, 2012), the groups characterized this signal pathway in these cells using immunofluorescence, electron microscopy and qPCR techniques. One particularly interesting experiment to show that the hedgehog pathway was functional in these cells was to add the hedgehog agonist, SAG (Smoothened agonist), and then to isolate the cells for immunofluorescence at different times.

Gradually, one can see the appearance of the smoothened protein into the cilium as indicated by increasing intensity of the immunofluorescence staining. Conversely, patched levels in the cilium, decreased. This is a hallmark of hedgehog activation (Fig. 2).
Fig. 2 copiaFig. 2 Immunofluorescence micrographs of hESC showing smoothened (green), acetylated tubulin (red) and DAPI (blue). The micrographs from left to right represents SAG treatments at t = 0, 1 and 4 hours.

However, an additional interesting observation was made concerning these stem cells. An important characteristic for stem cells is the presence of certain transcription factors which render these cells in the pluripotent or undifferentiated state. These include Oct4, Sox2, and Nanog whose localization had been observed in the nucleus as expected for other TFs.

However, the Danish groups curiously found a subpopulation of stem cells where these TFs were additionally localized to the primary cilium (Fig. 3). This had never been observed or investigated before.  Additionally, proper negative controls were  carried out to exclude this phenomenon from being an artifact (e.g. bleed through).
Fig. 3 copia Fig. 3 Stem cell markers (Sox2, Nanog, and Oct4) localizing to the nucleus and the primary cilia (arrows) of hESC line LRB003. This and the previous figure show shifted overlay images whereby the green and red channels have been slightly shifted so that the red channel doesn’t swamp out the intensity of the green channels.

Thus, it raises an intriguing possibility that perhaps the primary cilia plays a previously uncharacterized role in the differentiation/proliferation state of the hESCs via possible modifications of these TFs perhaps analogous to the processing of the Gli transcription factors (Hui and Angers, 2011). Another curious observation is that the subpopulation of cells whose primary cilia are positive for these TFs always occur in clusters which might hint at its mechanistic explanation.  In conclusion, since stem cells are now being more routinely used for regenerative medicine such as repair of severed spinal cord (Lu et al. 2012), it behooves us to better learn the molecular mechanisms that keeps these invaluable cells in an undifferentiated state so that we can harness their full therapeutic potential.

REFERENCES

Awan A, Oliveri RS, Jensen PL, Christensen ST, Andersen CY. 2010 Immunoflourescence and mRNA analysis of human embryonic stem cells (hESCs) grown under feeder-free conditions. Methods Mol Biol. 584:195-210.

Bernhardt M, Galach M, Novak D, Utikal J. 2012 Mediators of induced pluripotency and their role in cancer cells – current scientific knowledge and future perspectives. Biotechnol J. 7:810-821.

Cerdan C, Bhatia M. 2010 Novel roles for Notch, Wnt and Hedgehog in hematopoesis derived from human pluripotent stem cells. Int J Dev Biol. 54:955-963.

Han YG, Spassky N, Romaguera-Ros M, Garcia-Verdugo JM, Aguilar A, Schneider-Maunoury S, Alvarez-Buylla A. 2008 Hedgehog signaling and primary cilia are required for the formation of adult neural stem cells.Nat Neurosci. 11:277-284.

Hui CC, Angers S. 2011 Gli proteins in development and disease. Annu Rev Cell Dev Biol. 27:513-537.

Kiprilov EN, Awan A, Desprat R, Velho M, Clement CA, Byskov AG, Andersen CY, Satir P, Bouhassira EE, Christensen ST, Hirsch RE 2008 Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery. J Cell Biol. 2008 180:897-904.

Lu P, Wang Y, Graham L, McHale K, Gao M, Wu D, Brock J, Blesch A, Rosenzweig ES, Havton LA, Zheng B, Conner JM, Marsala M, Tuszynski MH. 2012 Long-distance growth and connectivity of neural stem cells after severe spinal cord injury. Cell 150:1264-73.

Nielsen SK, Møllgård K, Clement CA, Veland IR, Awan A, Yoder BK, Novak I, Christensen ST. 2008 Characterization of primary cilia and Hedgehog signaling during development of the human pancreas and in human pancreatic duct cancer cell lines. Dev Dyn. 237:2039-52.

Pazour GJ, Dickert BL, Vucica Y, Seeley ES, Rosenbaum JL, Witman GB, Cole DG. 2000 Chlamydomonas IFT88 and its mouse homologue, polycystic kidney disease gene tg737, are required for assembly of cilia and flagella. J Cell Biol 151: 709-18.

Pedersen LB, Veland IR, Schrøder JM, Christensen ST. 2008 Assembly of primary cilia. Dev Dyn. 237:1993-2006.

Veland IR, Awan A, Pedersen LB, Yoder BK, Christensen ST. 2009 Primary cilia and signaling pathways in mammalian development, health and disease. Nephron Physiol. 111: 39-53.

Discovery of Primary Cilia in Stem Cells.

//

Sorting stem cells

In ALL ARTICLES, SCIENCE & STEM CELLS on January 4, 2013 at 9:13 am

I’d like to see these techniques utilized to separate adult stem cells in order to determine if there is a bio-availability factor which can be isolated. dg

https://i1.wp.com/blog.pagerduty.com/wp-content/uploads/sorting-lego.jpg

Sorting stem cellsAmerican Institute of Physics

When an embryonic stem cell is in the first stage of its development it has the potential to grow into any type of cell in the body, a state scientists call undifferentiated. A team of researchers from Scotland has now demonstrated a way to easily distinguish undifferentiated embryonic stem cells from later-stage stem cells whose fate is sealed. The results are published in the American Institute of Physics’ (AIP) journal Biomicrofluidics.

The researchers used an electric field to pull stem cells through a fluid in a process called dielectrophoresis. They varied the frequency of the voltage used to generate the electric field and studied how the cells moved, a response that was affected by the cell’s electrical properties. The researchers found that differentiated stem cells could store a significantly greater charge on their outer membranes, a characteristic that might be used to effectively identify and separate them from undifferentiated cells. The researchers write that the wrinkling, folding, and thinning of a cell’s membrane as it differentiates may explain why the later-stage cells can store more charge. The sorting method may prove useful in separating cells for biomedical research or ultimately for treatments of diseases such as Parkinson’s.

###

Article: “Dielectrophoresis based discrimination of human embryonic stem cells from differentiating derivatives” is published in the journal Biomicrofluidics.

Link: http://bmf.aip.org/resource/1/biomgb/v6/i4/p044113_s1

Authors: Srinivas Velugotla (1), Steve Pells (2), Heidi K. Mjoseng (2), Cairnan R. E. Duffy (2), Stewart Smith (1), Paul De Sousa (2) and Ronald Pethig (1).

(1) Institute for Integrated Micro and Nano Systems, School of Engineering, The University of Edinburgh, Scotland (2) Centre for Regenerative Medicine, College of Medicine and Veterinary Medicine, The University of Edinburgh, Scotland

via Sorting stem cells.

CHRISTOPHER REEVE’S STEM CELL SPINAL CORD MISTAKE

In CELEBRITIES & STEM CELLS, STEM CELLS IN THE NEWS on September 25, 2012 at 1:07 pm

spinal

A SAD BIRTHDAY FOR CHRISTOPHER REEVE

Christopher Reeve would most likely have reached his 60th birthday today had he focused on adult stem cell treatments instead of embryonic.

Adult stem cell treatments have produced significant therapeutic benefits in Spinal cord injury patients for years.  Patients have regained sensation in the extremities, bladder and bowel control and even the ability to walk.  It is sad that a misinformed or misguided and certainly limited focus on embryonic stem cells resulted in the loss of potential for this iconic actor to recover from spinal cord injury.

HISTORY OF STEM CELL TREATMENTS FOR SPINAL CORD INJURY

SPINAL CORD INJURY vs ASC HISTORY OF TREATMENT – http://tinyurl.com/SCIvsASC

REGENERATING THE CENTRAL NERVOUS SYSTEM  https://repairstemcell.wordpress.com/2011/10/17/regenerating-the-central-nervous-system/

Stepping Towards A Paralysis Cure, A Tale Of Two Supermen Stem Cells Cure 23 Year Old Male of Paralysis – C6…-C7 injury
https://repairstemcell.wordpress.com/2009/03/28/stepping-towards-a-paralysis-cure-a-tale-of-two-supermen-stem-cells-cure-23-year-old-male-of-paralysis-c6-c7-injury/

Paraplegic – Adult Stem Cell Success Stories – Laura Dominguez
https://repairstemcell.wordpress.com/2010/05/05/paraplegic-adult-stem-cell-success-stories-laura-dominguez/

PARALYZED COUSINS PLEASED WITH STEM CELL TREATMENT
https://repairstemcell.wordpress.com/2010/02/15/paralyzed-cousins-pleased-with-stem-cell-treatment/

Successful Stem Cell Treatment of Spinal Cord Injury in Dogs
https://repairstemcell.wordpress.com/2010/02/08/successful-stem-cell-treatment-of-spinal-cord-injury-in-dogs/

Spinal Cord Injury Patient Wins…and Loses
https://repairstemcell.wordpress.com/2010/02/08/spinal-cord-injury-patient-wins-and-loses/

STEM CELLS FOR SPINAL CORD INJURY
https://repairstemcell.wordpress.com/2009/12/31/stem-cells-for-spinal-cord-injury/

Adult Stem Cell Grafts Help Paralyzed Heal
https://repairstemcell.wordpress.com/2009/10/21/adult-stem-cell-grafts-help-paralyzed-heal/

Medical hope as paralysed dog cured by stem cell therapy – mirror.co.ukhttps://repairstemcell.wordpress.com/2009/10/08/medical-hope-as-paralysed-dog-cured-by-stem-cell-therapy-mirror-co-uk/

Major the Roseville police dog gets stem cell treatment – http://blogs.citypages.com/blotter/2011/01/major_police_dog_stem_cell.php

 

https://i2.wp.com/www.myhero.com/ReadingRoom/books/Christopher%20Reeve%20-%20Nothing%20Is%20Impossible.jpg

Christopher D’Olier Reeve[1] (September 25, 1952 – October 10, 2004) was an American actor, film director, producer, screenwriter, author and activist. He achieved stardom for his acting achievements, in particular his motion-picture portrayal of the fictional superhero Superman.

On May 27, 1995, Reeve became a quadriplegic after being thrown from a horse during an equestrian competition in Virginia. He required a wheelchair and breathing apparatus for the rest of his life. He lobbied on behalf of people with spinal-cord injuries and for human embryonic stem cell research, founding the Christopher Reeve Foundation and co-founding the Reeve-Irvine Research Center.[2]

STEM CELLS – MAGIC OR SCIENCE?

In STEM CELLS IN THE NEWS on July 26, 2012 at 11:28 am

 

A friend asked:

“I would like nothing more than to beleive adult stem cell working for different diseases, however I don’t. How can you take a sick cell and replant it and it becomes healthy.”

—————————————————————————

My answer:

HIGH TURNOVER RATE
This is not magic, it is biological fact. Let’s start with your body. “Just like us, cells grow old and die. When old cells die, new ones replace them. For example, a blood cell in our body lives for about 120 days. Another example is our skin cells. We shed our skin cells about every 35 days.” That’s the outer layer which is why tattoos fade over time and why tattoos go deep into the lower levels of skin.

I’M DYING! YES, WE ALL ARE…
So our cells are in a constant state of “getting older” or “getting sick” or moving towards “impaired function.” I’m dying says the soldier with the sucking chest wound. Yes, we all are, says his philosopher friend. And we are indeed. “He not busy being born is busy dying.” says Bob Dylan. Perhaps this is too philosophical but the point is this: our body and every one of the 5-50 trillion cells is either getting older and weaker and dying or is currently being born or repaired. It’s a dynamic entity, this shell we reside in and it is constantly changing.

GETTING DOWN WITH THE SICKNESS
If you have too many cells with impaired function, especially in a specific area, which are damaged, necrotic, not getting enough nutrients, minerals etc and are getting exposure to too many toxins, inflammation, infections, etc which it can not eliminate, then something will go wrong and you will get sick with the capital ‘S’ and it is time to call in the workers to fix you up.

BOB THE BUILDER HAS SOME COMPETITION
Stem cells are the body’s construction workers. They do both renovations and they do ground up construction. Renovations amount to taking dead tissue and cells – necrotic – and re-energizing them so they come back to life (no zombie jokes please). This can be seen in hundreds of heart studies and trials and thousands of congestive heart failure patients where necrotic heart tissue implanted with stem cells was found to be living and beating a few weeks later. Ground up construction is where they set up shop on a blank field and build something new. This can be seen when stem cells create mini bypasses where stents were implanted. They are actually smart enough to know the “stent area” is a dangerous heavy traffic area and even if the stent is working, they will create offramps and onramps around the stent or bypasses with capillaries. Pretty cool huh?

YOU CAN’T ALWAYS GET WHAT YOU WANT
Not magic, just plain old science and if your body was able to produce enough stem cells to run to the heart, it could do it by itself. In fact, it is trying, desperately to do exactly that but the body in congestive heart failure is like a single mother with 6 kids, 3 jobs and 2 dogs standing on one foot and juggling chain saws. She just can’t do it all, she is stretched to the limits of her endurance, something has to give…and it does. So while your body is sending stem cells to the heart, and the feet and the pancreas, liver, kidney, brain, endocrine and lymphatic and circulatory system infrastructure, RIGHT NOW, to renovate and build new cells and tissues, it is not sending ENOUGH and the fact that our single mom smokes and lives near a factory and does other people’s laundries, exposing herself to multiple chemical toxins, doesn’t sleep much, can only afford McD’s and is highly stressed, etc etc just taxes her body all the more.

RIGHT HERE, RIGHT NOW
So YOU have stem cells in YOUR body RIGHT NOW which are running around, differentiating into different cell types and healing you. All the time. So while our bodies are in a constant state of degradation, our stem cells are constantly fighting that degradation.

WANT THE SCIENCE?
If you would like trials and studies to back this up there are about 2,600 at last count and I can refer you to some that address a specific condition.

 

Embryonic Pluripotency May Give Up Secrets – But So What?

In STEM CELLS IN THE NEWS on July 20, 2012 at 4:07 am

More forward looking statements (without any of the standard legalese on risks, assumptions and uncertainty) regarding the great mystery of embryonic stem cells maybe, sort of, perhaps, soon to give up it’s secrets.

So what’s the deal? 

“Pluripotency is defined as the capacity of individual cells to initiate all lineages of the mature organism in response to signals from the embryo or cell culture environment.”1

Embryonic stem cells have pluripotency.  Discovery of the methods and aspects which allow for pluripotency in embryonic stem cells are definitely a research milestone and one which will advance many lines of inquiry in various fields of medicine…but…there are a few major issues, particularly in the USA.

  • There are already ADULT stem cells which ARE PLURIPOTENT and can be used for treatment. 
  • The US is in a bitter tug of war in both accurate media coverage of stem cells and research and use of adult versus embryonic stem cells. 
  • Pharma is trying to make drugs out of this stuff and get patents on it.
  • In most cases, your own body can supply the necessary stem cells for your own treatment. 
  • And of course, the controversy issues of utilizing embryonic stem cells which will cause the religious right to exert huge resistance on any advancement of embryonic stem cell research in the US.

While the US is still playing a game of catch up in adult and embryonic research and especially treatment; please remember, embryonic stem cell research was fully funded with government backing in many countries around the world for over a decade resulting in…

ZERO EMBRYONIC STEM CELL TREATMENTS.

So don’t get your hopes up for embryonic treatment anytime soon, but then again,

SO WHAT!

ADULT stem cells are here, now, powerful, safe and effective.  What are you waiting for?

Mechanisms That Allow Embryonic Stem Cells to Become Any Cell in the Human Body Identified

ScienceDaily (July 18, 2012) — New research at the Hebrew University of Jerusalem sheds light on pluripotency — the ability of embryonic stem cells to renew themselves indefinitely and to differentiate into all types of mature cells. Solving this problem, which is a major challenge in modern biology, could expedite the use of embryonic stem cells in cell therapy and regenerative medicine.

If scientists can replicate the mechanisms that make pluripotency possible, they could create cells in the laboratory which could be implanted in humans to cure diseases characterized by cell death, such as Alzheimer’s, Parkinson’s, diabetes and other degenerative diseases.

To shed light on these processes, researchers in the lab of Dr. Eran Meshorer, in the Department of Genetics at the Hebrew University’s Alexander Silberman Institute of Life Sciences, are combining molecular, microscopic and genomic approaches. Meshorer’s team is focusing on epigenetic pathways — which cause biological changes without a corresponding change in the DNA sequence — that are specific to embryonic stem cells.

The molecular basis for epigenetic mechanisms is chromatin, which is comprised of a cell’s DNA and structural and regulatory proteins. In groundbreaking research performed by Shai Melcer, a PhD student in the Meshorer lab, the mechanisms which support an “open” chromatin conformation in embryonic stem cells were examined. The researchers found that chromatin is less condensed in embryonic stem cells, allowing them the flexibility or “functional plasticity” to turn into any kind of cell.

A distinct pattern of chemical modifications of chromatin structural proteins (referred to as the acetylation and methylation of histones) enables a looser chromatin configuration in embryonic stem cells. During the early stages of differentiation, this pattern changes to facilitate chromatin compaction.

But even more interestingly, the authors found that a nuclear lamina protein, lamin A, is also a part of the secret. In all differentiated cell types, lamin A binds compacted domains of chromatin and anchors them to the cell’s nuclear envelope. Lamin A is absent from embryonic stem cells and this may enable the freer, more dynamic chromatin state in the cell nucleus. The authors believe that chromatin plasticity is tantamount to functional plasticity since chromatin is made up of DNA that includes all genes and codes for all proteins in any living cell. Understanding the mechanisms that regulate chromatin function will enable intelligent manipulations of embryonic stem cells in the future.

“If we can apply this new understanding about the mechanisms that give embryonic stem cells their plasticity, then we can increase or decrease the dynamics of the proteins that bind DNA and thereby increase or decrease the cells’ differentiation potential,” concludes Dr. Meshorer. “This could expedite the use of embryonic stem cells in cell therapy and regenerative medicine, by enabling the creation of cells in the laboratory which could be implanted in humans to cure diseases characterized by cell death, such as Alzheimer’s, Parkinson’s, diabetes and other degenerative diseases.”

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